iFATS | Site Wide Activity

iFats wrote a new blog post: Adipocyte-derived factors suppress heart contraction.

Sat, 04 Sep 2010 08:16:01 +0200

iFats wrote a new blog post: Adipocyte-derived factors suppress heart contraction. Background:Obesity is strongly associated with cardiovascular diseases including systemic hypertension, coronary artery disease and heart failure. Despite several investigations the pathophysiological mechanisms involved remain unclear. We have previously shown that adipose tissue exerts a highly potent activity with an acute depressant effect on cardiomyocytes, thus suggesting direct involvement of adipose tissue in the development of [...]

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iFats wrote a new blog post: Differentiation of human multipotent dermal fibroblasts into islet-like cell clusters.

Sat, 04 Sep 2010 07:59:35 +0200

iFats wrote a new blog post: Differentiation of human multipotent dermal fibroblasts into islet-like cell clusters. BACKGROUND: We have previously obtained a clonal population of cells from human foreskin that is able to differentiate into mesodermal, ectodermal and endodermal progenies. It is of great interest to know whether these cells could be further differentiated into functional insulin-producing cells. RESULTS: Sixty-one single-cell-derived dermal fibroblast clones were established from human foreskin by limiting [...]

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iFats wrote a new blog post: Geometric control of human stem cell morphology and differentiation.

Sat, 04 Sep 2010 07:58:07 +0200

iFats wrote a new blog post: Geometric control of human stem cell morphology and differentiation. During tissue morphogenesis, stem cells and progenitor cells migrate, proliferate, and differentiate, with striking changes in cell shape, size, and acting mechanical stresses. The local cellular function depends on the spatial distribution of cytokines as well as local mechanical microenvironments in which the cells reside. In this study, we controlled the organization of human adipose [...]

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Vincent became a registered member

Fri, 03 Sep 2010 15:57:47 +0200

Vincent became a registered member

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iFats wrote a new blog post: Simple Modular Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and Prevascularization.

Fri, 03 Sep 2010 07:56:06 +0200

iFats wrote a new blog post: Simple Modular Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and Prevascularization. Large-scale tissue engineering is limited by nutrient perfusion and mass transport limitations, especially oxygen diffusion, which restrict construct development to smaller than clinically relevant dimensions and limit the ability for in vivo integration. The goal of this work was to develop a modular approach to tissue engineering, where scaffold and tissue size, transport issues, and [...]

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iFats wrote a new blog post: Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord.

Fri, 03 Sep 2010 07:52:29 +0200

iFats wrote a new blog post: Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord. Up to 2.8 x 10(7) fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical cord (UC) using a simple and highly reproducible explant culture approach. Cells derived from whole UC, similar to cells collected [...]

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iFats wrote a new blog post: A Subset of Osteoblasts Expressing High Endogenous Levels of PPARgamma Switches Fate to Adipocytes in the Rat Calvaria Cell Culture Model.

Fri, 03 Sep 2010 07:46:46 +0200

iFats wrote a new blog post: A Subset of Osteoblasts Expressing High Endogenous Levels of PPARgamma Switches Fate to Adipocytes in the Rat Calvaria Cell Culture Model. BACKGROUND: Understanding fate choice and fate switching between the osteoblast lineage (ObL) and adipocyte lineage (AdL) is important to understand both the developmental inter-relationships between osteoblasts and adipocytes and the impact of changes in fate allocation between the two lineages in normal aging and certain diseases. The goal of this study was to determine when [...]

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Giuseppe became a registered member

Thu, 02 Sep 2010 07:51:53 +0200

Giuseppe became a registered member

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iFats wrote a new blog post: Id4, a new candidate gene for senile osteoporosis, acts as a molecular switch promoting osteoblast differentiation.

Thu, 02 Sep 2010 07:44:25 +0200

iFats wrote a new blog post: Id4, a new candidate gene for senile osteoporosis, acts as a molecular switch promoting osteoblast differentiation. Excessive accumulation of bone marrow adipocytes observed in senile osteoporosis or age-related osteopenia is caused by the unbalanced differentiation of MSCs into bone marrow adipocytes or osteoblasts. Several transcription factors are known to regulate the balance between adipocyte and osteoblast differentiation. However, the molecular mechanisms that regulate the balance between adipocyte and osteoblast differentiation in [...]

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iFats wrote a new blog post: Tissue engineering and the use of stem/progenitor cells for airway epithelium repair.

Thu, 02 Sep 2010 07:42:29 +0200

iFats wrote a new blog post: Tissue engineering and the use of stem/progenitor cells for airway epithelium repair. Stem/progenitor cells can be used to repair defects in the airway wall, resulting from e.g., tumors, trauma, tissue reactions following long-time intubations, or diseases that are associated with epithelial damage. Several potential sources of cells for airway epithelium have been identified. These can be divided into two groups. The first group consists of endogenous progenitor [...]

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iFats wrote a new blog post: Ochratoxin A inhibits adipogenesis through the ERK-PPAR-gamma pathway in human adipose tissue-derived mesenchymal stem cells.

Thu, 02 Sep 2010 07:41:07 +0200

iFats wrote a new blog post: Ochratoxin A inhibits adipogenesis through the ERK-PPAR-gamma pathway in human adipose tissue-derived mesenchymal stem cells. chratoxin A (OTA) is a ubiquitous fungal metabolite with nephrotoxic, carcinogenic and apoptotic potential. Although the toxic effects of OTA in various cell types are well characterized, it is not known whether OTA has an effect on stem cell differentiation. In this study, we demonstrate that OTA inhibits adipogenesis in human adipose tissue-derived mesenchymal stem [...]

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Kacey became a registered member

Wed, 01 Sep 2010 14:13:29 +0200

Kacey became a registered member

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iFats wrote a new blog post: Isolating adipose-derived mesenchymal stem cells from lipoaspirate blood and saline fraction.

Wed, 01 Sep 2010 07:39:44 +0200

iFats wrote a new blog post: Isolating adipose-derived mesenchymal stem cells from lipoaspirate blood and saline fraction. Isolation of adipose-derived stem cells (ASCs) typically involves 8+ hours of intense effort, requiring specialized equipment and reagents. Here, we present an improved technique for isolating viable populations of mesenchymal stem cells from lipoaspirate saline fractions within 30 minutes. Importantly, the cells exhibit remarkable similarities to those obtained using the traditional isolation protocols, in terms [...]

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iFats wrote a new blog post: Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype.

Wed, 01 Sep 2010 07:37:39 +0200

iFats wrote a new blog post: Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype. The ability to isolate, expand and differentiate adult stem cells into a chondrogenic lineage is an important step in the development of tissue engineering approaches for cartilage repair or regeneration for the treatment of joint injury or osteoarthritis, as well as for their application in plastic or reconstructive surgery. Adipose-derived stem cells (ASCs) provide an [...]

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iFats wrote a new blog post: Neural potential of adipose stem cells.

Wed, 01 Sep 2010 07:35:39 +0200

iFats wrote a new blog post: Neural potential of adipose stem cells. In the last few years, adipose tissue, which has been largely ignored by anatomists and physicians for centuries, has found new brightness thanks to the stem cells contained within. These adipose derived stem cells (ADSC) have the same characteristics of the mesenchymal stem cells (MSC) residing in bone marrow. They have the same cell surface [...]

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iFats wrote a new blog post: Influence of Chondrocytes on the Chondrogenic Differentiation of Adipose Stem Cells.

Tue, 31 Aug 2010 13:30:48 +0200

iFats wrote a new blog post: Influence of Chondrocytes on the Chondrogenic Differentiation of Adipose Stem Cells. In the current study, whether or not chondrogenic differentiation of ASCs could be enhanced by soluble factors from or co-culture with chondrocytes was determined. In vitro pellet cultures were carried out in five ways using ASCs or chondrocytes in passage 3, as follows: #1, 2.5 x 105 ASCs were cultured in DMEM/F-12 supplemented with 1% [...]

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iFats wrote a new blog post: Development of extracellular matrices mimicking stepwise adipogenesis of mesenchymal stem cells.

Tue, 31 Aug 2010 13:30:39 +0200

iFats wrote a new blog post: Development of extracellular matrices mimicking stepwise adipogenesis of mesenchymal stem cells.

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iFats wrote a new blog post: Reprogramming of Human Umbilical Cord Stromal Mesenchymal Stem Cells for Myogenic Differentiation and Muscle Repair.

Tue, 31 Aug 2010 13:30:11 +0200

iFats wrote a new blog post: Reprogramming of Human Umbilical Cord Stromal Mesenchymal Stem Cells for Myogenic Differentiation and Muscle Repair. Human umbilical cord stromal mesenchymal stem cells (hUCS-MSCs) have the potential to differentiate into numerous cell types including epithelial cells, neurons and hepatocytes in vitro, in addition to mesenchyme-derived cells such as osteocytes, chondrocytes and adipocytes. One important property of these cells is the lack of type II major histocompatibility complex class molecules, thus allowing [...]

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iFats wrote a new blog post: Cyclic Tensile Culture Promotes Fibroblastic Differentiation of Marrow Stromal Cells Encapsulated in Poly(Ethylene Glycol)-Based Hydrogels.

Mon, 30 Aug 2010 13:30:53 +0200

iFats wrote a new blog post: Cyclic Tensile Culture Promotes Fibroblastic Differentiation of Marrow Stromal Cells Encapsulated in Poly(Ethylene Glycol)-Based Hydrogels. To inform future efforts in tendon/ligament tissue engineering, our laboratory has developed a well-controlled model system with the ability to alter both external tensile loading parameters and local biochemical cues to better understand marrow stromal cell differentiation in response to both stimuli concurrently. In particular, the synthetic, poly(ethylene glycol)-based hydrogel material oligo(poly(ethylene glycol) fumarate) (OPF) [...]

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iFats wrote a new blog post: Multiple mesodermal lineage differentiation of Apodemus sylvaticus embryonic stem cells in vitro.

Mon, 30 Aug 2010 13:30:51 +0200

iFats wrote a new blog post: Multiple mesodermal lineage differentiation of Apodemus sylvaticus embryonic stem cells in vitro. BACKGROUND: Embryonic stem (ES) cells have attracted significant attention from researchers around the world because of their ability to undergo indefinite self-renewal and produce derivatives from the three cell lineages, which has enormous value in research and clinical applications. Until now, many ES cell lines of different mammals have been established and studied. In addition, [...]

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