Background:Obesity is strongly associated with cardiovascular diseases including  systemic hypertension, coronary artery disease and heart failure. Despite several investigations the pathophysiological mechanisms involved remain unclear. We have previously shown that adipose tissue exerts a highly potent activity with an acute depressant effect on cardiomyocytes, thus suggesting direct involvement of  adipose tissue in the development of [...]

   Authors

Look C; Morano I; Ehrhart-Bornstein M; Bornstein SR; Lamounier-Zepter V

   Institution

Clinical Medicine III, University of Technology, Dresden, Germany.

Read more

BACKGROUND: We have previously obtained a clonal population of cells from human foreskin that is able to differentiate into mesodermal, ectodermal and endodermal progenies. It is of great interest to know whether these cells could be further differentiated into functional insulin-producing cells. RESULTS: Sixty-one single-cell-derived dermal fibroblast clones were established from human foreskin by limiting [...]

   Authors

Bi D; Chen FG; Zhang WJ; Zhou GD; Cui L; Liu W; Cao Y

   Institution

Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 200011, China. blue_150@163.com

Read more

 During tissue morphogenesis, stem cells and progenitor cells migrate, proliferate, and differentiate, with striking changes in cell shape, size, and acting mechanical stresses. The local cellular function depends on the spatial distribution of cytokines as well as local mechanical microenvironments in which  the cells reside. In this study, we controlled the organization of human adipose  [...]

   Authors

Wan LQ; Kang SM; Eng G; Grayson WL; Lu XL; Huo B; Gimble J; Guo XE; Mow VC; Vunjak-Novakovic G

   Institution

Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904 New York, NY 10027, USA. gv2131@columbia.edu.

Read more

Large-scale tissue engineering is limited by nutrient perfusion and mass transport limitations, especially oxygen diffusion, which restrict construct development to smaller than clinically relevant dimensions and limit the ability  for in vivo integration. The goal of this work was to develop a modular approach  to tissue engineering, where scaffold and tissue size, transport issues, and [...]

   Authors

Lovett M; Rockwood D; Baryshyan A; Kaplan DL

   Institution

Department of Biomedical Engineering, Tufts University , Medford, Massachusetts.

Read more

Up to 2.8 x 10(7) fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical cord (UC) using a simple and highly reproducible explant culture approach. Cells derived from whole UC, similar to cells collected [...]

   Authors

Majore I; Moretti P; Stahl F; Hass R; Kasper C

   Institution

Institute of Technical Chemistry, Leibniz University of Hannover, Callinstrasse 5, Hannover, 30167, Germany, IngridaMajore@aol.com.

Read more

BACKGROUND: Understanding fate choice and fate switching between the osteoblast lineage (ObL) and adipocyte lineage (AdL) is important to understand both the developmental inter-relationships between osteoblasts and adipocytes and the impact of changes in fate allocation between the two lineages in normal aging and certain diseases. The goal of this study was to determine when [...]

   Authors

Yoshiko Y; Oizumi K; Hasegawa T; Minamizaki T; Tanne K; Maeda N; Aubin JE

   Institution

Department of Oral Growth and Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.

Read more

Excessive accumulation of bone marrow adipocytes observed in senile osteoporosis  or age-related osteopenia is caused by the unbalanced differentiation of MSCs into bone marrow adipocytes or osteoblasts. Several transcription factors are known to regulate the balance between adipocyte and osteoblast differentiation. However, the molecular mechanisms that regulate the balance between adipocyte and osteoblast differentiation in [...]

   Authors

Tokuzawa Y; Yagi K; Yamashita Y; Nakachi Y; Nikaido I; Bono H; Ninomiya Y; Kanesaki-Yatsuka Y; Akita M; Motegi H; Wakana S; Noda T; Sablitzky F; Arai S; Kurokawa R; Fukuda T; Katagiri T; Schonbach C; Suda T; Mizuno Y; Okazaki Y

   Institution

Division of Functional Genomics and Systems Medicine, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Saitama,

Read more

Stem/progenitor cells can be used to repair defects in the airway wall, resulting from e.g., tumors, trauma, tissue reactions following long-time intubations, or diseases that are associated with epithelial damage. Several potential sources of cells for airway epithelium have been identified. These can be divided into two groups. The first group consists of endogenous progenitor [...]

   Authors

Roomans GM

   Institution

School of Health and Medical Sciences, Orebro University, Orebro University Hospital, SE-70185 Orebro, Sweden. godfried.roomans@oru.se

Read more

chratoxin A (OTA) is a ubiquitous fungal metabolite with nephrotoxic, carcinogenic and apoptotic potential. Although the toxic effects of OTA in various cell types are well characterized, it is not known whether OTA has an effect on stem cell differentiation. In this study, we demonstrate that OTA inhibits adipogenesis in human adipose tissue-derived mesenchymal stem [...]

   Authors

Lim S; Jang HJ; Kim JK; Kim JM; Park EH; Yang JH; Kim YH; Yea K; Ryu SH; Suh PG

   Institution

UNIST, School of Nano-Biotechnology & Chemical Engineering, Ulsan, Korea, Republic of; anguqaos@postech.ac.kr.

Read more

Isolation of adipose-derived stem cells (ASCs) typically involves 8+ hours of intense effort, requiring specialized equipment and reagents. Here, we present an improved technique for isolating viable populations of mesenchymal stem cells from lipoaspirate saline fractions within 30 minutes. Importantly, the cells exhibit remarkable similarities to those obtained using the traditional isolation protocols, in terms [...]

   Authors

Francis MP; Sachs PC; Elmore LW; E Holt S

Read more